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What is Paper Chromatography: Principle, Types & Uses
Paper chromatography is one of the types of chromatography procedures which runs on a piece of specialized paper. It is a planar chromatography systems wherein a cellulose filter paper acts as a stationary phase on which separation of compounds...
26 commentsHPLC analysis:principle, theory-Video tutorial of hplc-chromatography
HPLC is an indispensable research analysis tool in the field of clinical, industrial and scientific research. The theory and principle of analysis in HPLC is based on separation of components from the substance under analysis.
31 commentsWhat is chromatography: Principle, method & types
Chromatography is a non-destructive procedure for resolving a complex mixture into its individual fractions or compounds. This is a separation procedure and the separated entities are identified by other analytical techniques like UV-visible,...
11 commentsHPLC instrumentation: Video demo of HPLC system
HPLC instrumentation has basically a pump, column, injector, detector and recorder.
6 commentsTLC thin layer chromatography: Principle, Procedure
TLC chromatography or Thin layer chromatography is a type of planar chromatography. TLC is routinely used by researcher in the field of phyto-chemicals, biochemistry etc. to identify the components in a compound mixture like alkaloids,...
19 commentsHPLC degasser techniques: Types of solvent degassing
In HPLC degassing of solvents, mobile phase is very essential for proper operation and retrieval of uniform data. Degassing removes entrapped air bubbles from the solvents. Improper degassing leads to many problems like fluctuation in pressure,...
0 commentsHPLC detector types: Principle in their working
HPLC detectors constitute an important accessories of HPLC instrument. This part of HPLC helps in detection and identification of compounds in the sample injected. These detectors are designed to have certain properties like ♦ Being inert...
19 commentsColumn chromatography: principle, procedure & applications
Column chromatography is the actual or the basic type of chromatography procedure which was developed during early stages of chromatography discovery. From it the principle of chromatography is described and these are still followed in the most...
1 commentIon exchange chromatography: Principle, method & applications
Ion exchange chromatography is a process of separation of molecules of like or same charges from a mixture by use of ion exchange resins. Principle: The separation occurs by reversible exchange of ions between the ions present in the solution and...
0 commentsHPTLC-High performance thin layer chromatography
HPTLC is High Performance Thin layer Chromatography or High Pressure Thin Layer Chromatography. This is a sophisticated advancement in Thin Layer chromatography (TLC). It has added advantages like better resolution, faster development of spots...
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